Endocrine Abstracts

Members of the Euphorbia L. genus have been used both in both traditional and alternative medicine for treatment of a wide range of disorders since time immemorial. Due to a wide spectrum of compounds of phoyphenolic nature, they are known to show anti-oxidant, anti-inflammatory, antimicrobial, fungicidal and antileukemic effects. The work was initiated to study a hypoglycemic effect of polyphenolic compounds isolated from two types of the Euphorbia L. genus. Polyphen...

Causing production of free radicals and reduction of antioxidant system activity, in the pancreatic β-cells, in particular, resulting in reduction in insulin production and consequently to hyperglycemia, oxidative stress could be a pathogenetic mechanism of morbidity in diabetes mellitus. Search for and development of medicines with antioxidant and hypoglycemic activity remains urgent.Aim: The work was initiated to study antioxidant and hypoglycemic...

Obesity is considered as a major factor of insulin resistance and diabetes mellitus risk. Study on lipid composition of tissues in obesity is relevant in understanding of mechanisms underlying insulin resistance.Aim: The work was initiated to study lipid composition of liver and skeletal muscles tissues in rats with alimentary obesity.Materials and methods: Obesity was induced in rats leading an inactive lifestyle with prolonged hi...

Aim: The work was initiated to study TNF-α gene polymorphism and parameters of oxidative stress in patients with type 2 diabetes mellitusMaterials and methods: We examined 41 healthy subjects and 20 patients with diabetes mellitus to study G308A (rs1800629) polymorphism in TNF-α gene by means of allele-specific PCR method (AS-PCR). Rates of lipid peroxidation (LP) were determined by concentration of malon dialdehyde (MDA); catalase activity was...

According to the findings from the recent studies, the oxidative stress can be a cause for type 2 diabetes mellitus onset. In that context, the development of drugs with antioxidant effect to be used for prevention and treatment of the disease is of vital importance. The work was initiated to study the oxidative stress in the experimental diabetes and methods of its correction by means of natural antioxidants. In our study we used polyphenols isolated from the seeds of common ...

Disturbance in insulin signaling under effect of sphingolipids is thought to be the mechanism underlying the obesity-mediated insulin resistance. The work was initiated to study sphingolipid metabolism in tissues of rats with obesity induced by a prolonged high-calorie diet. The lipid extraction and purification was performed by Folch’s method; concentrations of sphingosine and ceramide were measured by Lauter et al. method. In obesity, the levels of total gangli...

The work was initiated to study some biochemical parameters in peripheral blood of animals upon diet-induced obesity and insulin resistance modeling. Obesity was induced in BALB/c mice by keeping them on the high- carbon diet for 13 weeks. The commercially available kits were used to assay glucose, alkaline phosphatase, creatine kinase and cholesterol; concentrations of testosterone were measured by the enzyme immunoassay. The high carbon diet in BALB/c mice served as a basis ...

Background: In Pathogenesis of Neurodegeneration in Diabetes Mellitus (DM) addition to insulin resistance, glycemic control and blood plasma lipid levels existing other mechanisms were proposed. We investigated plasma insulin level in patients according to severity of diabetic neuropathy.Materials and methods: In 32 people with DM2 glycemia, HbA1c, blood plasma insulin, blood total cholesterol, triglycerides, Low Density Lipoproteins (LDLP) levels were m...

Associated with the increased generation of free radicals and reduced activity of antioxidant system, oxidative stress is a pathogenetic mechanism underlying the onset of diabetic complications. Antioxidants are known to facilitate normalization of lipid peroxidation, composition, structures and functions of biological membranes. Antioxidants are universal membrane-trophic agents protecting against damaging factors. Plant antioxidants play a significant role in treatment of di...

Obesity of varying severities can be seen in the vast majority of patients with type 2 diabetes mellitus (DM2). Oxidative stress is considered as a key pathogenetic link in the formation of metabolic disorders in obesity. The work was initiated to assess intensity of oxidative stress in obese patients with DM2.Materials and methodsThe patients were divided into 3 groups. Patients with DM2 and BMI > 30 kg/m2 were incl...